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Effects of Heat-treatment on the Stability and Composition of Metabolomic Extracts from the Earthworm Eisenia fetida

Published

Author(s)

Tracey B. Schock, Sheri Strickland, Edna J. Steele, Daniel W. Bearden

Abstract

Environmental metabolomics studies employing earthworms as sentinels for soil contamination are numerous, but the instability of the metabolite extracts from these organisms has been minimally addressed. This study evaluated the efficacy of adding a heat- treatment step in two commonly used extraction protocols (Bligh and Dyer and D2O phosphate buffer) as a pre-analytical stabilization method. The resulting metabolic profiles of Eisenia fetida were assessed using principal component analysis and spectral evaluations. The heated Bligh and Dyer extractions produced stabilized profiles with minimal variation of the extracted metabolomic profiles over time, providing a more suitable method for metabolomic analysis of earthworm extracts.
Citation
Metabolomics

Keywords

Earthworm, Eisenia fetida, Heat Stabilization, Metabolomics, NMR, Stability, Tissue Extraction

Citation

Schock, T. , Strickland, S. , Steele, E. and Bearden, D. (2016), Effects of Heat-treatment on the Stability and Composition of Metabolomic Extracts from the Earthworm Eisenia fetida, Metabolomics (Accessed April 12, 2024)
Created February 13, 2016, Updated January 27, 2020