Schock, T.
, Strickland, S.
, Steele, E.
and Bearden, D.
(2016),
Effects of Heat-treatment on the Stability and Composition of Metabolomic Extracts from the Earthworm Eisenia fetida, Metabolomics
(Accessed September 9, 2024)
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.
Effects of Heat-treatment on the Stability and Composition of Metabolomic Extracts from the Earthworm Eisenia fetida
Published
Author(s)
, Sheri Strickland, Edna J. Steele,
Abstract
Environmental metabolomics studies employing earthworms as sentinels for soil contamination are numerous, but the instability of the metabolite extracts from these organisms has been minimally addressed. This study evaluated the efficacy of adding a heat- treatment step in two commonly used extraction protocols (Bligh and Dyer and D2O phosphate buffer) as a pre-analytical stabilization method. The resulting metabolic profiles of Eisenia fetida were assessed using principal component analysis and spectral evaluations. The heated Bligh and Dyer extractions produced stabilized profiles with minimal variation of the extracted metabolomic profiles over time, providing a more suitable method for metabolomic analysis of earthworm extracts.Citation
Metabolomics
Pub Type
Journals
Keywords
Earthworm, Eisenia fetida, Heat Stabilization, Metabolomics, NMR, Stability, Tissue Extraction
Citation
Issues
If you have any questions about this publication or are having problems accessing it, please contact reflib@nist.gov.
Created February 13, 2016, Updated January 27, 2020