Microstructures of biomedical scaffolds can greatly affect cellular activities in tissue regeneration. In particular, scaffolds used in bone tissue regeneration should have several microstructural characteristics, including high porosity, appropriate pore size and shape, and permeability or pore interconnectivity. In addition, they should have high mechanical properties and be biodegradable and biocompatible. In this respect, the solid freeform fabricated PCL/-TCP scaffold has been widely applied in bone tissue regeneration. In this study, using PCL/-TCP material, we designed PCL/-TCP (20 mass %) scaffolds with various offset values so that the fabricated scaffolds showed two different pore sizes that were dependent on the offset value (0 %, 25 %, 50 %, 75 %, and 100 %), which showed very similar porosity (about 62 %), a square pore shape, and 100% pore interconnectivity. The fabricated scaffolds were assessed not only for physical properties, including calculated bending modulus and water-uptake ability, but also for biological capabilities by culturing osteoblast-like cells (MG63) for various offset values of the scaffolds. Compared with the no-offset scaffolds, the offset scaffolds (especially those with offset values of 50 % and 100 %) showed superior physical and biological improvements, such as higher bending modulus (maximum increase of 7 %), cell-seeding efficiency, cell viability (increase of about 60 %), ALP activity (increase of about 50 %), and calcium deposition. Based on these results, we conclude that the offset scaffold can be a potential biomaterial for bone tissue regeneration.
Pub Type: Journals
cell differentiation, biomaterial, polymer scaffold, tissue engineering