When culturing cells on flexible surfaces, it is important to consider extracellular matrix treatments that will remain on the surface under mechanical strain. Here we investigate differences in laminin surfaces that were deposited on oxidized PDMS (plasma only) versus oxidized PDMS treated with aminopropyltrimethoxysilane (silane-linked). We use specular X-ray reflectivity (SXR), transmission electron microscopy (TEM), and immunofluorescence to probe the quantity and uniformity of the laminin on the treated PDMS surfaces. We compare vascular smooth muscle cell (SMC) proliferation on laminin under equibiaxial strain between plasma only and silane-linked treatments.
Proceedings Title: American Chemical Society Division of Polymeric Materials: Science and Engineering Meeting| |Proceedings of the ACS Division of Polymeric Materials: Science & Engineering |ACS
Conference Dates: August 16-20, 2009
Conference Location: Washingont, DC
Pub Type: Conferences
specular X-ray reflectivity, transmission electron microscopy, laminin, PDMS, smooth muscle cell