DEVELOPING METROLOGY FOR TISSUE ENGINEERING: COLLINEAR OPTICAL COHERENCE AND CONFOCAL FLUORESCENCE MICROSCOPIES
Joy P. Dunkers, Marcus T. Cicerone, James Cooper, Forrest A. Landis, N Washburn
We present a novel application of optical coherence microscopy (OCM) by combining it with confocal fluorescence microscopy (CFM) to gather simultaneous structural and functional information on tissue engineered medical products (TEMPs) in a registered fashion. In this work, we describe the collinear OCM and CFM instrument. We demonstrate the utility of this dual-mode technique for TEMPs by comparing the images of fluorescently stained osteoblasts cultured in a polymeric TEMP for 21 days and 10 weeks.
Proceedings of the IEEE International Symposium on Biomedical Imaging
, Cicerone, M.
, Cooper, J.
, Landis, F.
and Washburn, N.
DEVELOPING METROLOGY FOR TISSUE ENGINEERING: COLLINEAR OPTICAL COHERENCE AND CONFOCAL FLUORESCENCE MICROSCOPIES, Proceedings of the IEEE International Symposium on Biomedical Imaging, Arlington, VA, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=854037
(Accessed December 8, 2023)