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DEVELOPING METROLOGY FOR TISSUE ENGINEERING: COLLINEAR OPTICAL COHERENCE AND CONFOCAL FLUORESCENCE MICROSCOPIES

Published

Author(s)

Joy P. Dunkers, Marcus T. Cicerone, James Cooper, Forrest A. Landis, N Washburn

Abstract

We present a novel application of optical coherence microscopy (OCM) by combining it with confocal fluorescence microscopy (CFM) to gather simultaneous structural and functional information on tissue engineered medical products (TEMPs) in a registered fashion. In this work, we describe the collinear OCM and CFM instrument. We demonstrate the utility of this dual-mode technique for TEMPs by comparing the images of fluorescently stained osteoblasts cultured in a polymeric TEMP for 21 days and 10 weeks.
Proceedings Title
Proceedings of the IEEE International Symposium on Biomedical Imaging
Conference Dates
May 15-18, 2004
Conference Location
Arlington, VA
Conference Title
ISBI 2004

Keywords

optical coherence tomography, fluorescence, scaffolds, osteoblasts

Citation

Dunkers, J. , Cicerone, M. , Cooper, J. , Landis, F. and Washburn, N. (2004), DEVELOPING METROLOGY FOR TISSUE ENGINEERING: COLLINEAR OPTICAL COHERENCE AND CONFOCAL FLUORESCENCE MICROSCOPIES, Proceedings of the IEEE International Symposium on Biomedical Imaging, Arlington, VA, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=854037 (Accessed July 13, 2024)

Issues

If you have any questions about this publication or are having problems accessing it, please contact reflib@nist.gov.

Created May 1, 2004, Updated February 19, 2017