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Determination of nicotine, NNN, and NNK in a new cigarette tobacco filler Standard Reference Material (SRM 3222) and its smoke



Walter B. Wilson, Jeanita Pritchett, Lane C. Sander


The United States has an annual production of approximately 800 million pounds of tobacco with estimated domestic sales of around 260 billion cigarettes/year. The National Institute of Standards and Technology (NIST) has recently collaborated with the Center for Tobacco Products at the Food and Drug administration (FDA) to develop a new low nicotine tobacco filler Standard Reference Material (SRM 3222). SRM 3222 was prepared from air-cured, low nicotine tobacco and is intended primarily for use in evaluating the accuracy of current and new analytical procedures for measuring nicotine, N-nitrosonornicotine (NNN), 4-(methylnitrosamino)-1-(3- pyridyl-1-butanone (NNK), and moisture in cigarettes sold daily. The certified values for nicotine, NNN, and NNK are based on the results of measurements made by NIST, U.S. Centers for Disease Control and Prevention (CDC), and commercial laboratories using isotope dilution liquid chromatography with tandem mass spectrometry (ID-LC-MS/MS) with different sample preparation techniques. Briefly, 0.250 g portions of finely ground SRM 3222 were vortex in a 15 mL polypropylene tubes with either 10 mL of 100 mM aqueous ammonium acetate or 5 mL of methanol extraction solution for a total of 1 h and 18 h, respectively. In the case of the smoke analysis, preliminary studies have been conducted using gas chromatography/MS to identify nicotine in the tobacco smoke collected from burning approximately 10 g of SRM 3222
Proceedings Title
71st Tobacco Science Research Conference
Conference Dates
September 17-20, 2017
Conference Location
Bonita Springs, FL


Tobacco, Standard Reference Materials, Tobacco Smoke Condensate, Nicotine, Tobacco Specific Nitrosamines


Wilson, W. , Pritchett, J. and Sander, L. (2018), Determination of nicotine, NNN, and NNK in a new cigarette tobacco filler Standard Reference Material (SRM 3222) and its smoke, 71st Tobacco Science Research Conference, Bonita Springs, FL, [online], (Accessed April 15, 2024)
Created January 1, 2018, Updated January 29, 2019