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Determination of Functional Collective Motions in a Protein at Atomic Resolution using Coherent Neutron Scattering

Published

Author(s)

Liang Hong, NItin Jain, Xiaolin Cheng, Ana Bernal, Madhu Sudan Tyagi, Jeremy C. Smith

Abstract

Protein function often depends on global, collective internal motions. However, the simultaneous quantitative experimental determination of the forms, amplitudes and timescale of these motions has remained elusive. Here, we demonstrate that a complete description of these large-scale dynamic modes can be obtained using coherent neutron scattering experiments on perdeuterated samples. Using this approach, a microscopic relationship between the structure, dynamics and function in a protein, cytochrome P450cam, is established. The approach developed here should be of general applicability to protein systems.
Citation
Science Advances
Volume
2
Issue
10

Keywords

Protein dynamics, neutron scattering

Citation

Hong, L. , Jain, N. , Cheng, X. , Bernal, A. , Tyagi, M. and Smith, J. (2016), Determination of Functional Collective Motions in a Protein at Atomic Resolution using Coherent Neutron Scattering, Science Advances, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=921815 (Accessed February 26, 2024)
Created October 13, 2016, Updated October 12, 2021