Determination of Fat-Soluble Vitamins and Carotenoids in Standard Reference Material 3280 Multivitamin/Multielement Tablets by Liquid Chromatography with Absorbance Detection
Jeanice M. Brown Thomas, Katherine E. Sharpless, Catherine A. Rimmer, James H. Yen
The concentrations of selected fat-soluble vitamins and carotenoids in Standard Reference Material (SRM) 3280 Multivitamin/Multielement Tablets have been determined by two independent liquid chromatographic methods with measurements performed by the National Institute of Standards and Technology (NIST). This SRM has been prepared as part of a collaborative effort between NIST and the National Institutes of Healths Office of Dietary Supplements (NIH/ODS). The SRM is also intended to support the Dietary Supplement Ingredient Database that is being established by the U.S. Department of Agriculture. The methods used at NIST to determine the concentration levels of vitamin A, E, and β-carotene in the SRM employed reversed-phase liquid chromatography with absorbance detection. The relative precision of these methods ranged from 2 % to 8 % for the analytes measured. SRM 3280 is primarily intended for use in validating analytical methods for the determination of selected vitamins, carotenoids, and elements in multivitamin/multielement tablets and similar matrices.
, Sharpless, K.
, Rimmer, C.
and Yen, J.
Determination of Fat-Soluble Vitamins and Carotenoids in Standard Reference Material 3280 Multivitamin/Multielement Tablets by Liquid Chromatography with Absorbance Detection, Journal of Aoac International
(Accessed February 28, 2024)