Determination of Caffeine, and Caffeine-Related Metabolites in Ephedra-Containing Standard Reference Materials Using Liquid Chromatography With Absorbance Detection and Tandem Mass Spectrometry

Published

April 24, 2007

Author(s)

Jeanice M. Brown Thomas, Katherine E. Sharpless, S. Mitvalsky, Mark Roman, James H. Yen, Mary B. Satterfield

Abstract

The concentration of caffeine and caffeine-related compounds in two ephedra-containing reference materials have been determined by three independent methods with measurements performed by the National Institute of Standards and Technology (NIST) and a collaborating laboratory. Results from the three methods were used for value assignment of caffeine, theobromine, and theophylline in these SRMs. The methods used at NIST to determine the concentration levels of caffeine, theobromine, and theophylline in SRM 3243 Ephedra-Containing Solid Oral Dosage Form and SRM 3244 Ephedra-Containing Protein Powder employed reversed-phase liquid chromatography with absorbance detection and tandem mass spectrometry. These reference materials are part of the first suite in a series of NIST Standard Reference Materials (SRMs) that provide concentration values for multiple components in dietary supplements. These SRMs are primarily intended for method validation and for use as control materials to support the analysis of dietary supplements and similar materials.

Citation

Journal of Aoac International

Volume

90

Pub Type

Journals

Keywords

caffeine, ephedra-containing reference materials, liquid chromatography, tandem mass spectrometry, theobromine, theophylline

Citation

Brown, J. , Sharpless, K. , Mitvalsky, S. , Roman, M. , Yen, J. and Satterfield, M. (2007), Determination of Caffeine, and Caffeine-Related Metabolites in Ephedra-Containing Standard Reference Materials Using Liquid Chromatography With Absorbance Detection and Tandem Mass Spectrometry, Journal of Aoac International (Accessed October 14, 2025)