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Crystal Structure of the YchF Protein Reveals Binding Sites for GTP and Nucleic Acid



A Teplyakov, G Obmolova, S Y. Chu, J Toedt, E Eisenstein, A J. Howard, G L. Gilliland


The bacterial protein encoded by the gene ychF is 1 of 11 universally conserved GTPases, and the only one whose function is unknown. The crystal structure determination of YchF was sought to help with the the functional assignment of the protein. The YchF protein from Haemophilus influenzae was cloned, and expressed, and the crystal structure was determined at 2.4- resolution. The polypeptide chain is folded into three domains. The N-terminal domain has a mononucleotide binding fold typical for the P-loop NTPases. An 80-residue domain next to it has a pronounced α-helical coiled coil. The c-terminal domain features a six-stranded half-barrel that curves around an α-helix. The crablike three-domain structure of YchF suggests the binding site for a double-stranded nucleic acid in the cleft between the domains. The structure of the putative GTP-binding site is consistent with the postulated guanine specificity of the protein. Fluorescence measurements have demonstrated the ability of YchF to bind a double-stranded nucleic acid and GTP. Taken together with other experimental data and genomic analysis, these results suggest that YchF may be part of a nucleoprotein complex and may function as a GTP-dependent translation factor.
Journal of Bacteriology


colied coil, crystal structure, GTPase, haemophilus influenzae, P-loop NTPase, RNA-binding, structural geonomics, TGS domain, translation factor


Teplyakov, A. , Obmolova, G. , Chu, S. , Toedt, J. , Eisenstein, E. , Howard, A. and Gilliland, G. (2003), Crystal Structure of the YchF Protein Reveals Binding Sites for GTP and Nucleic Acid, Journal of Bacteriology (Accessed June 12, 2024)


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Created July 1, 2003, Updated February 19, 2017