Controlled Vesicle Self-Assembly in Continuous Two Phase Flow Microfluidic Channels
Andreas Jahn, Wyatt N. Vreeland, Laurie E. Locascio, Michael Gaitan
The spontaneous self-assembly of phospholipids into liposomes in aqueous solution results in the encapsulation of reagents into quantized packets in a manner that mimics cellular life-processes. Since their discovery in 1965, liposomes have been used in a variety of biological applications including targeted delivery of chemotherapeutic drugs and DNA vectors, or as model systems for the study of biological membranes to name a few. Traditional liposome preparation methods are all conducted through mixing of bulk phases, leading to inhomogeneous chemical and/or mechanical conditions during liposome formation, hence liposomes are often polydisperse in size and lamellarity. Here we show formation of liposomes that encapsulate reagents in a continuous 2-phase flow planar microfluidic network with precision control of size, over the range of 100 to 300 nm, by manipulation of liquid flow rates. We demonstrate that by creating a solvent-aqueous interfacial region in a microfluidic format that is homogenous and controllable on the length scale of a liposome facilitates fine control of liposome size and polydispersity.
Proceedings of Hilton Head 2004, A Solid-State Sensor, Actuator, and Microsystem Workshop
June 6-10, 2004
Hilton Head Island, SC, USA
Hilton Head 2004, A Solid-State Sensor, Actuator, and Microsystem Workshop
, Vreeland, W.
, Locascio, L.
and Gaitan, M.
Controlled Vesicle Self-Assembly in Continuous Two Phase Flow Microfluidic Channels, Proceedings of Hilton Head 2004, A Solid-State Sensor, Actuator, and Microsystem Workshop, Hilton Head Island, SC, USA, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=31518
(Accessed August 9, 2022)