Controlled Encapsulation of a Hydrophilic Drug Simulant in Nano-Liposomes Using Continuous Flow Microfluidics
Andreas Jahn, Joseph E. Reiner, Wyatt N. Vreeland, Don DeVoe, Laurie E. Locascio, Michael Gaitan
A new method to tailor the size and size distribution of nanometer scale liposomes and control loading of liposomes with a model drug in a continuous-flow microfluidic design is presented. Size and size dispersion are determined with tandem Asymmetric Flow Field Flow Fractionation (AF4) and Multi-Angle Laser Light Scattering (MALLS). Fluorescence Correlation Spectroscopy (FCS) combined with Fluorescence Cumulant Analyis (FCA) allow for determining the number of encapsulated molecules . Results show that this system allows for control of loading efficiency as well as minimization of encapsulant consumption.
11th Annual Nano Science and Technology Institute (NSTI) Nanotech 2008
, Reiner, J.
, Vreeland, W.
, DeVoe, D.
, Locascio, L.
and Gaitan, M.
Controlled Encapsulation of a Hydrophilic Drug Simulant in Nano-Liposomes Using Continuous Flow Microfluidics, 11th Annual Nano Science and Technology Institute (NSTI) Nanotech 2008, Boston, MA, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=32987
(Accessed July 3, 2022)