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PUBLICATIONS

Comparison of Extraction Methods for the Analysis of Ginkgo biloba Leaves and Ginkgo-Containing Tablets

Published

Author(s)

S. Howerton, Kate Rimmer, Lane C. Sander

Abstract

In preparation for the certification of three Standard Reference Materials containing Ginkgo biloba, three extraction techniques are compared. For the three flavonoid aglycones (quercetin, isorhamnetin, and kaempferol) Soxhlet extraction and pressurized fluid extraction are quantitatively compared using an internal standard approach. In addition the hydrolysis of the glycosides to the flavonoid aglycones is reported as a function of acid concentration and time. For the terpene trilactones (Ginkolides A, B, C, J and bilobalide) Soxhlet extraction, sonication, and pressurized fluid extraction are quantitatively compared using an internal standard approach. The data demonstrate that the two methods for the aglycones are statistically equivalent, and that the optimal hydrolysis conditions require reflux with 25% HCl (mass fraction) for three hours. The data also indicate that Soxhlet extraction and sonication are the preferred methods for the quantitative determination of the terpene trilactones.
Citation
Journal of Chromatography A
Pub Type
Journals

Keywords

aglycones, extraction, Ginkgo biloba, terpene trilactones

Citation

Howerton, S. , Rimmer, K. and Sander, L. (2021), Comparison of Extraction Methods for the Analysis of Ginkgo biloba Leaves and Ginkgo-Containing Tablets, Journal of Chromatography A (Accessed October 10, 2025)

Issues

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Created October 12, 2021
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