NOTICE: Due to a lapse in annual appropriations, most of this website is not being updated. Learn more.

Form submissions will still be accepted but will not receive responses at this time. Sections of this site for programs using non-appropriated funds (such as NVLAP) or those that are excepted from the shutdown (such as CHIPS and NVD) will continue to be updated.

U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PUBLICATIONS

Comparison of Extraction Methods for the Analysis of Ginkgo biloba Leaves and Ginkgo-Containing Tablets

Published

Author(s)

S. Howerton, Kate Rimmer, Lane C. Sander

Abstract

In preparation for the certification of three Standard Reference Materials containing Ginkgo biloba, three extraction techniques are compared. For the three flavonoid aglycones (quercetin, isorhamnetin, and kaempferol) Soxhlet extraction and pressurized fluid extraction are quantitatively compared using an internal standard approach. In addition the hydrolysis of the glycosides to the flavonoid aglycones is reported as a function of acid concentration and time. For the terpene trilactones (Ginkolides A, B, C, J and bilobalide) Soxhlet extraction, sonication, and pressurized fluid extraction are quantitatively compared using an internal standard approach. The data demonstrate that the two methods for the aglycones are statistically equivalent, and that the optimal hydrolysis conditions require reflux with 25% HCl (mass fraction) for three hours. The data also indicate that Soxhlet extraction and sonication are the preferred methods for the quantitative determination of the terpene trilactones.
Citation
Journal of Chromatography A
Pub Type
Journals

Keywords

aglycones, extraction, Ginkgo biloba, terpene trilactones

Citation

Howerton, S. , Rimmer, K. and Sander, L. (2021), Comparison of Extraction Methods for the Analysis of Ginkgo biloba Leaves and Ginkgo-Containing Tablets, Journal of Chromatography A (Accessed October 17, 2025)

Issues

If you have any questions about this publication or are having problems accessing it, please contact [email protected].

Created October 12, 2021
Was this page helpful?