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Combined scanning probe and light scattering characterization of multi-stage self-assembly of targeted liposome-based delivery systems

Published

Author(s)

Natalia Farkas, John A. Dagata, Chengli Yang, Antonina Rait, Kathleen Pirollo, Esther Chang

Abstract

The mean size and size distribution of a targeted nanoparticle delivery system (NDS) strongly influences the intrinsic stability and functionality of this molecular complex, affects its performance as a systemic drug delivery platform, and ultimately determines its efficacy towards early detection and treatment of cancer. Since its components undergo significant reorganization during multiple stages of self-assembly, it is essential to monitor size and stability of the complex throughout NDS formulation in order to assure its potency and manufacturability prior to entering clinical trials. This work combines scanning probe microscopy (SPM) and dynamic light scattering (DLS) techniques to obtain quantitative and reliable size measurements of NDS and to investigate how variations in NDS formulation or self-assembly process impact the size, structure and functionality of the complex with various therapeutic and diagnostic agent payloads. These combined SPM and DLS methods, when implemented at an early stage of NDS formulation, present a potential measurement approach to facilitate drug discovery and development, optimization and quality control during manufacturing of NDS.
Citation
Measurement Science and Technology
Volume
22

Keywords

targeted delivery systems, scanning probe microscopy, dynamic light scattering, magnetic contrast agent, liposome

Citation

Farkas, N. , Dagata, J. , Yang, C. , Rait, A. , Pirollo, K. and Chang, E. (2010), Combined scanning probe and light scattering characterization of multi-stage self-assembly of targeted liposome-based delivery systems, Measurement Science and Technology, [online], https://doi.org/10.1088/0957-0233/22/2/024006 (Accessed April 23, 2024)
Created December 21, 2010, Updated February 7, 2019