Chemically-Sensitive Bioimaging with Coherent Raman Scattering
Charles H. Camp, Marcus T. Cicerone
Raman scattering provides an intrinsic fingerprint of chemical composition. Spontaneous Raman spectroscopy has been used for many decades to interrogate biological materials and systems, but is not widely used for imaging due to relatively slow signal acquisition. Coherent Raman imaging (CRI) technologies have evolved over the last 15 years to provide the same rich chemical information but orders-of-magnitude faster. CRI methods are now poised to begin emerging as widely used tools for obtaining functional information in a label-free manner from biological systems. We briefly review the development and application of these methods.