Sander, L.
, Rimmer, C.
and Wilson, W.
(2020),
Characterization of triacontyl (C-30) liquid chromatographic columns, Journal of Chromatography A, [online], https://doi.org/10.1016/j.chroma.2019.460732
(Accessed April 25, 2024)
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.
Characterization of triacontyl (C-30) liquid chromatographic columns
Published
Author(s)
, ,
Abstract
Differences in the performance characteristics of seventeen commercial C30 liquid chromatographic columns were studied for the separation of carotenoid isomers. A mixture consisting of nine xanthophyll and hydrocarbon carotenoids were separated under conditions carefully chosen to reveal changes in selectivity. The influence of the mobile phase modifier, column temperature, and mobile phase flow rate were evaluated. Shape recognition was characterized with Standard Reference Material (SRM) 869b Column Selectivity Test Mixture, for correlation with carotenoid retention behavior. Regular changes were observed across a broad spectrum of shape selectivity characteristics as indicated by SRM 869b. Better separations of carotenoid isomers were achieved with C30 columns than were possible with C18 columns, even after optimization of separation conditions.Citation
Journal of Chromatography A
Pub Type
Journals
Download Paper
Keywords
C30 column, shape selectivity, triacontyl column, carotenoids, SRM 869b, column characterization
Citation
Created March 15, 2020, Updated April 27, 2020