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Characterization of NIST Food-Matrix Standard Reference Materials for their Vitamin C Content



Jeanice M. Brown Thomas, James H. Yen, Katherine E. Sharpless


The vitamin C concentrations in three food-matrix Standard Reference Materials (SRMs) from the National Institute of Standards and Technology (NIST) have been determined by liquid chromatography (LC) with absorbance detection. These materials (SRM 1549a Whole Milk Powder, SRM 1849a Infant/Adult Nutritional Formula, and SRM 3233 Fortified Breakfast Cereal) have been characterized to support analytical measurements made by food processors that are required to provide information about their products’ vitamin C content on the labels of products distributed in the United States. The SRMs are primarily intended for use in validating analytical methods for the determination of selected vitamins, elements, fatty acids, and other nutrients in these materials and in similar matrixes. They can also be used for quality assurance in the characterization of test samples or in-house control materials, and for establishing measurement traceability. Relative precision of the LC method used to measure vitamin C in the food-matrix SRMs characterized in this study ranged from 4 % to 7.5 %.
Analytical and Bioanalytical Chemistry


ascorbic acid, food, reference material, standard reference material, liquid chromatography, absorbance detection


Brown, J. , Yen, J. and Sharpless, K. (2013), Characterization of NIST Food-Matrix Standard Reference Materials for their Vitamin C Content, Analytical and Bioanalytical Chemistry, [online], (Accessed April 14, 2024)
Created March 26, 2013, Updated November 10, 2018