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Biomarkers of Oxidative DNA Damage in Tissue Engineered Skin Measured by LC/MS and GC/MS Technologies

Published

Author(s)

H Rodriguez, M Birincioglu, C D. O'Connell, Peter E. Barker, Pawel Jaruga, M. Dizdaroglu

Abstract

Tissue engineering is an emerging area of biotechnology that will provide replacement tissues for patients, as well as complex, functional biological systems for research and testing in the pharmaceutical industry. Fundamentals to many tissue-engineered devices are issues of inflammation associated with how biological cells respond to a given matrix or when inserted into the body. The goal of the research was to help identify cellular biomarkers and assess measurement technologies that will help manufacturers determine how cells react to inflammatory stimuli from different polymer matrixes and assure that tissue-engineered medical products are free of genetic changes that could occur during the manufacturing phase, storage or shipment of the product. The use of gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS) technology was used to quantitatively measure the level of oxidative DNA damage (a marker if cellular inflammation) in tissue engineered skin. No significant level of damage was detected in the tissue engineered skin compared to control cells.
Proceedings Title
Southern Biomedical Engineering Conference | 21st | | Medical and Engineering Publishers
Conference Dates
September 1, 2002
Conference Title
Southern Biomedical Engineering Conference

Keywords

inflammation, oxidative DNA damage, tissue engineered skin

Citation

Rodriguez, H. , Birincioglu, M. , O'Connell, C. , Barker, P. , Jaruga, P. and Dizdaroglu, M. (2002), Biomarkers of Oxidative DNA Damage in Tissue Engineered Skin Measured by LC/MS and GC/MS Technologies, Southern Biomedical Engineering Conference | 21st | | Medical and Engineering Publishers (Accessed April 13, 2024)
Created September 1, 2002, Updated February 17, 2017