Alperovich, N.
, Ross, D.
and Scott, B.
(2023),
Automation Protocol for High-Efficiency and High-Quality Genomic DNA Extraction from Saccharomyces cerevisiae, Plos One, [online], https://doi.org/10.1371/journal.pone.0292401, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=936466 (Accessed May 20, 2026)
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Automation Protocol for High-Efficiency and High-Quality Genomic DNA Extraction from Saccharomyces cerevisiae
Published
Author(s)
, , Benjamin Scott
Abstract
Although many protocols have been previously developed for genomic DNA (gDNA) extraction from S. cerevisiae, to take advantage of recent advances in laboratory automation and DNA-barcode sequencing, there is a need for automated methods that can provide high-quality gDNA at high efficiency. Here, we describe and demonstrate a fully automated method that avoids the use of hazardous reagents (e.g., phenol, chloroform), glass beads for mechanical cell disruption, or incubation of samples at 100 °C (i.e., boiling). We show that our protocol can extract gDNA with high efficiency both from cells grown in liquid culture and from colonies grown on agar plates. We also show results from gel electrophoresis that demonstrate that the resulting gDNA is of high quality.Citation
Plos One
Pub Type
Journals
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Keywords
laboratory automation, synthetic biology, Saccharomyces Cerevisiae, DNA extraction
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Created October 17, 2023, Updated May 18, 2026