Assessment of extracellular vesicles purity using proteomic standards

Published

September 26, 2017

Author(s)

Tingting Wang, Kyle Anderson, Illarion Turko

Abstract

The increasing interest in extracellular vesicles (EVs) research is fueled by reports indicating their unique role in intercellular communication and potential connection to the development of common human diseases. The unique role assumes unique protein and nucleic acid cargo. Unfortunately, accurate analysis of EVs cargo faces a challenge of EVs isolation. Generally used isolation techniques do not separate different subtypes of EVs and even more, poorly separate EVs from non-EVs contaminants. Development of EVs isolation protocols urgently needs a quantitative method of EVs purity assessment. We report here that multiple reaction monitoring assay using internal standards carrying peptides for quantification of EVs and non-EVs proteins is a suitable approach to assess purity of EVs preparations. As a first step in potential standardization of EVs isolation, we have evaluated polymer-based precipitation techniques and compared them to traditional centrifugation protocols.

Citation

Analytical Chemistry

Volume

89

Issue

20

Pub Type

Journals

Download Paper

https://doi.org/10.1021/acs.analchem.7b03119

Local Download

Citation

Wang, T. , Anderson, K. and Turko, I. (2017), Assessment of extracellular vesicles purity using proteomic standards, Analytical Chemistry, [online], https://doi.org/10.1021/acs.analchem.7b03119, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=924014 (Accessed April 16, 2024)