Subrata Mishra (Fed)

Subrata is part of the Biomolecular Structure and Function Group in the Biomolecular Measurement Division at MML. He works as a Research Chemist and is based at the Institute for Bioscience and Biotechnology Research (IBBR). His background is in experimental biophysics and NMR spectroscopy. He earned his degree at Georgia State University, where his research centered on understanding the structure and thermodynamics of RNA targeting zinc finger proteins. After that, he conducted post-doctoral research at Johns Hopkins University, where he developed NMR methods for structure elucidation of large proteins, and investigated how structural dynamics choreograph function in an important class of antibiotic producing mega-dalton enzymes – nonribosomal peptide synthetases. Before joining NIST, he contributed his expertise at United States Pharmacopeia, where he worked on developing NMR methods for characterizing drugs, ranging from small molecules to biologics.

His current research at NIST/IBBR aims to translate our understanding of biomolecular dynamics into real-world applications – specifically in the creation of biomolecular measurement reagents in the next-gen protein sequencing project by leveraging directed-evolution guided by NMR. His overarching goal extends beyond this project, as he envisions that these research findings can provide a template for biosensor design. His second area of research focus is on NMR method development, where he is interested in developing newer hyper-fast NMR data acquisition schemes for multi-dimensional NMR experiments.