Extreme expression of DNA repair protein apurinic/apyrimi-dinic endonuclease I (APE1) in human breast cancer as measured by liquid-chromatography isotope-dilution tandem mass spectrometry

Published

September 15, 2015

Author(s)

Erdem Coskun, Pawel Jaruga, Prasad T. Reddy, Miral M. Dizdar

Abstract

Apurinic/apyrimidinic endonuclease 1 (APE1) is a DNA re-pair protein and plays other important roles. Increased lev-els of APE1 in cancer have been reported. However, avail-able methods to measure APE1 levels are indirect and not quantitative. We previously developed an approach using LC-MS/MS with isotope-dilution to accurately measure APE1. Here, we applied this methodology to measure APE1 levels in normal and cancerous human breast tissues. Ex-treme expression of APE1 in malignant tumors was ob-served, suggesting that breast cancer cells may require APE1 for survival. Accurate measurement of APE1 may be essential to develop novel treatment strategies and APE1 inhibitors as drugs.

Citation

Biochemistry

Volume

54

Issue

38

Pub Type

Journals

Download Paper

https://doi.org/10.1021/acs.biochem.5b00928

Local Download

Keywords

DNA repair, apurinic/apyrimi-dinic endonuclease I (APE1), breast cancer, LC-MS/MS

Citation

Coskun, E. , Jaruga, P. , Reddy, P. and Dizdar, M. (2015), Extreme expression of DNA repair protein apurinic/apyrimi-dinic endonuclease I (APE1) in human breast cancer as measured by liquid-chromatography isotope-dilution tandem mass spectrometry, Biochemistry, [online], https://doi.org/10.1021/acs.biochem.5b00928, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=919226 (Accessed October 9, 2025)