Photopatterned Polyelectrolyte Multilayers for Cell Culture

Published

November 9, 2006

Author(s)

Darwin Reyes-Hernandez, J. Brian Hutchison, Laurie E. Locascio, Michael Gaitan

Abstract

Patterned two-dimensional cell cultures are increasingly important for constructing bioanalytical tools for cell and molecular biology to address questions about interactions, signaling, migration, and differentiation, among others. This contribution presents a unique method for patterning layer-by- layer polymer with dissimilar cell attachment characteristics. Polyelectrolyte multilayers (PEMs) were deposited and exposed with UV light through a binary photomask to form a patterned upper crosslinked stratum where cells are not viable and a lower cytophilic stratum. Specifically, bilayers of polyacrylic acid and polyacrylamide (PAA/PAAm) were patterned (i.e., crosslinked) on top of electrostatically bound layers of biocompatible poly(allylamine hydrochloride)/poly(sodium 4-styrenesulfonate (PAH/PSS) bilayers. This work demonstrates the facile patterning of PEMs for two-dimensional cell cultures that exhibit natural phenotypic expression and long-term viability, which are significant advantages over many other cell patterning strategies.

Proceedings Title

Proceedings of Micro Total Analysis Systems

Conference Dates

November 5-9, 2006

Conference Location

Tokyo, 1, JA

Conference Title

The 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (?TAS2006)

Pub Type

Conferences

Keywords

cell culture, coating, photo polymerization, polymer, surface modification

Citation

Reyes-Hernandez, D. , Hutchison, J. , Locascio, L. and Gaitan, M. (2006), Photopatterned Polyelectrolyte Multilayers for Cell Culture, Proceedings of Micro Total Analysis Systems, Tokyo, 1, JA (Accessed October 21, 2025)