Jones, B.
, Vergne, M.
, Bunk, D.
, Locascio, L.
and Hayes, M.
(2021),
Reagentless Cleavage of the Peptide Backbone Using Light-Generated Radicals, Science
(Accessed October 21, 2025)
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Reagentless Cleavage of the Peptide Backbone Using Light-Generated Radicals
Published
Author(s)
Barbara J. Jones, , , , M A. Hayes
Abstract
A key step for protein identification and characterization is cleavage into distinct fragments. All current cleavage methods require the addition of reagents, either proteolytic enzymes or chemical agents, and often a second reagent to discontinue cleavage. We have developed a selective cleavage process for peptides and proteins that employs light-generated radicals produced from titanium dioxide. These short-lived radicals can be produced in confined regions and time scales, making this technique highly tunable. By shining light on titanium dioxide, under a variety of conditions and morphologies, in the presence of peptides and proteins in solution, selective cleavage is consistently observed at proline residues.Citation
Science
Pub Type
Journals
Keywords
cleavage, hydroxyl radical, peptide backbone, titanium dioxide
Citation
Issues
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Created October 12, 2021