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PUBLICATIONS

New Certified Reference Material for the Standardization of Human Cardiac Troponin I Assays

Published

Author(s)

David M. Bunk, Michael J. Welch

Abstract

Background: To address the continuing need for the standardization of clinical human cardiac troponin I (cTnI) assays, NIST, with the assistance of the AACC/IFCC Cardiac Troponin I Standardization Committee, has developed a new reference material, standard reference material (SRM) 2921: human cardiac troponin complex.Methods: The concentration of cTnI in SRM 2921 was determined through a combination of reversed phase HPLC with UV detection and amino acid analysis. The characterization of the intact troponin subunits was accomplished through reversed phase HPLC coupled with mass spectrometry (HPLC/MS). Post-translational modifications to the cTnI in SRM 2921 were investigated by combining proteolytic digestion with matrix assisted laser desorption/ionization mass spectrometry(MALDI-MS).Results: A certified concentration value of human cTnI in SRM 2921 was determined through a method that provides traceability to the SI. Additionally, reference concentration values for cTnT and cTnC are also provided.Conclusions: The development of the first cTnI reference material should greatly assist in the international harmonization of cTnI assays.
Citation
Clinical Chemistry
Pub Type
Journals

Keywords

HPLC, mass spectrometry, protein, troponin

Citation

Bunk, D. and Welch, M. (2008), New Certified Reference Material for the Standardization of Human Cardiac Troponin I Assays, Clinical Chemistry (Accessed April 25, 2024)

Created October 16, 2008