Balss, K.
, Ross, D.
, Begley, H.
, Olsen, K.
and Tarlov, M.
(2008),
Controlled Mixing: An in situ Hybridization Assay of Peptide Nucleic Acids with DNA by Temperature Gradient Focusing, Journal of the American Chemical Society
(Accessed April 19, 2024)
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Controlled Mixing: An in situ Hybridization Assay of Peptide Nucleic Acids with DNA by Temperature Gradient Focusing
Published
Author(s)
, , H Begley, K G. Olsen,
Abstract
An in situ nucleic acid hybrdization assay involving DNA and peptide nucleic acids (PNAs) employs temperature gradient focusing to separate and concentrate DNA from PNA/DNA hybrids. The assay is demonstrated in a capillary format but easily incorporated into a lab-on-a-chip format. The fluorescent labeled PNA probe was mixed in situ with a complementary and noncomplementary single-stranded DNA target. The hybridization assay performed by TGF represents a qualitatively new method for mixing in microfluidic formats.Citation
Journal of the American Chemical Society
Pub Type
Journals
Keywords
concentration method, mixing, peptide nucleic acids (PNA), separations
Citation
Created October 16, 2008