Balss, K.
, Ross, D.
, Begley, H.
, Olsen, K.
and Tarlov, M.
(2008),
Controlled Mixing: An in situ Hybridization Assay of Peptide Nucleic Acids with DNA by Temperature Gradient Focusing, Journal of the American Chemical Society
(Accessed October 21, 2025)
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Controlled Mixing: An in situ Hybridization Assay of Peptide Nucleic Acids with DNA by Temperature Gradient Focusing
Published
Author(s)
, , H Begley, K G. Olsen,
Abstract
An in situ nucleic acid hybrdization assay involving DNA and peptide nucleic acids (PNAs) employs temperature gradient focusing to separate and concentrate DNA from PNA/DNA hybrids. The assay is demonstrated in a capillary format but easily incorporated into a lab-on-a-chip format. The fluorescent labeled PNA probe was mixed in situ with a complementary and noncomplementary single-stranded DNA target. The hybridization assay performed by TGF represents a qualitatively new method for mixing in microfluidic formats.Citation
Journal of the American Chemical Society
Pub Type
Journals
Keywords
concentration method, mixing, peptide nucleic acids (PNA), separations
Citation
Issues
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Created October 16, 2008