Analysis of Covalently Cationized Polystyrenes Using Liquid Chromatography and Mass Spectrometry

Published

January 1, 2004

Author(s)

Z Vakili, Kathleen M. Flynn, Charles M. Guttman, Mark Arnould, H M. Byrd

Abstract

Production of covalently charged polymers of known molecular mass is important in determining the ability of mass spectrometry (MS) to provide exact Molecular mass and molecular mass distribution information of synthetic polymers. Comparison of the mass spectra of polystyrene samples, ionized by both traditional metal cationization and covalent cationization reveals that covalent cationization increases signal-to-noise and provides more reproducible data. Covalently charged products are contaminated with excess reagents and side products and can be difficult to purify by precipitation. Purification is needed before further analysis can be carried out. Liquid chromatography (LC) is a widely used technique for organic separation and purification. MALDI-TOF-MS after LC is important in determining the presence of polymer in fractions collected from a column and provides insight into the separation efficiency.

Proceedings Title

ASMS Conference on Mass Spectrometry and Allied Topics | 52nd | Proceedings of the 52nd ASMS Conference on Mass Spectrometry and Allied Topics | American Society for Mass Spectrometry (ASMS)

Volume

80(5)

Conference Dates

May 23-27, 2005

Conference Location

Nashville, TN

Conference Title

ASMS Conference on Mass Spectrometry

Pub Type

Conferences

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Local Download

Keywords

liquid chromatography, polymer, purification, separation, synthesis

Citation

Vakili, Z. , Flynn, K. , Guttman, C. , Arnould, M. and Byrd, H. (2004), Analysis of Covalently Cationized Polystyrenes Using Liquid Chromatography and Mass Spectrometry, ASMS Conference on Mass Spectrometry and Allied Topics | 52nd | Proceedings of the 52nd ASMS Conference on Mass Spectrometry and Allied Topics | American Society for Mass Spectrometry (ASMS), Nashville, TN, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=852363 (Accessed October 15, 2025)