Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Nanopore-based Single Molecule Determination of Polymer-cation Binding and Membrane Protein Dynamics

Published

Author(s)

Joseph E. Reiner, John J. Kasianowicz, Joseph W. Robertson

Abstract

Polyethylene glycol (PEG) is a ubiquitous charge-neutral polymer having several useful properties. It has been used to estimate the diameter of functioning nanopores. In this talk I will describe how the detailed analysis of PEG induced current blockades from a single alpha hemolysin channel in an unsupported lipid bilayer can be used to estimate step-wise changes in the structure of a functioning alpha hemolysin nanopore to < 1 Å. I will also describe a thermodynamic model that estimates the binding strength of cations to single PEG molecules from both the magnitude of PEG induced current blockades and the mean residence time of PEG within the nanopore.

Citation

Reiner, J. , Kasianowicz, J. and Robertson, J. (2010), Nanopore-based Single Molecule Determination of Polymer-cation Binding and Membrane Protein Dynamics (Accessed March 29, 2024)
Created August 23, 2010, Updated October 12, 2021