Toxcity Screening of Halogenated Aliphatics Using a Novel In itro Volatile Chemical Exposure System

Published

April 20, 2001

Author(s)

K T. Geiss, J M. Frazier, D E. Dodd

Abstract

A major concern while conducting in vitro testing of volatile chemicals, such as halons or their alternatives, is loss of the test chemical during the course of experimental studies. Imprecision in dosimetry confounds experimental results and prevents appropriate comparisons among various chemicals and exposure concentrations. We designed a novel system for exposing cell cultures to volatiles and applied this technology to the toxicity evaluation of 20 halogenated aliphatics. To rank these chemicals, we then derived a composite toxicity index based on six separate in vitro toxicity assays. Our results show the ability to rank a group of chemicals, based on an in vitro toxicity index and the correlation of these results with published toxicity values. This provides a means of discerning relative toxic potencies among groups of chemicals exposed under controlled dosimetry conditions and is especially useful for ranking volatile chemicals in materials development programs.

Citation

Special Publication (NIST SP) - 984-4

Report Number

984-4

NIST Pub Series

Special Publication (NIST SP)

Pub Type

NIST Pubs

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Citation

Geiss, K. , Frazier, J. and Dodd, D. (2001), Toxcity Screening of Halogenated Aliphatics Using a Novel In itro Volatile Chemical Exposure System, Special Publication (NIST SP), National Institute of Standards and Technology, Gaithersburg, MD, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=909030 (Accessed October 28, 2025)