Determination of Fat-Soluble Vitamins and Carotenoids in Standard Reference Material 3280 Multivitamin/Multielement Tablets by Liquid Chromatography with Absorbance Detection

Published

August 17, 2011

Author(s)

Jeanice M. Brown Thomas, Katherine Sharpless, Catherine Rimmer, James Yen

Abstract

The concentrations of selected fat-soluble vitamins and carotenoids in Standard Reference Material (SRM) 3280 Multivitamin/Multielement Tablets have been determined by two independent liquid chromatographic methods with measurements performed by the National Institute of Standards and Technology (NIST). This SRM has been prepared as part of a collaborative effort between NIST and the National Institutes of Health's Office of Dietary Supplements (NIH/ODS). The SRM is also intended to support the Dietary Supplement Ingredient Database that is being established by the U.S. Department of Agriculture. The methods used at NIST to determine the concentration levels of vitamin A, E, and β-carotene in the SRM employed reversed-phase liquid chromatography with absorbance detection. The relative precision of these methods ranged from 2 % to 8 % for the analytes measured. SRM 3280 is primarily intended for use in validating analytical methods for the determination of selected vitamins, carotenoids, and elements in multivitamin/multielement tablets and similar matrices.

Citation

Journal of Aoac International

Volume

94

Issue

3

Pub Type

Journals

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Keywords

fat-soluble vitamins, carotenoids, dietary supplements, liquid chromatography, absorbance detection, mass spectrometry

Citation

Brown Thomas, J. , Sharpless, K. , Rimmer, C. and Yen, J. (2011), Determination of Fat-Soluble Vitamins and Carotenoids in Standard Reference Material 3280 Multivitamin/Multielement Tablets by Liquid Chromatography with Absorbance Detection, Journal of Aoac International, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=904001 (Accessed May 27, 2026)

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