We describe a new technique to segment and track the cell images of a breast cancer cell line in order to study cell migration and metastasis. Within each image cell phenotypes vary widely, ranging from very bright completely bounded cells to barely visible cells with little to no apparent boundaries. A series of different steps defines the different cell morphologies. Cell segmentation and cell tracking are done simultaneously. Several new methods are presented for removing background intensity, and defining the presence of barely visible cells, including a new method for background subtraction and the use of selective dilation to define brighter cell areas. We show results for initial cell growth.
Citation: Cancer Science
Pub Type: Journals
cancer cells, cell tracking, segmentation