Optimizing cell-material interactions is critical towards maximizing regeneration in tissue engineering. Combinatorial and high-throughput (CHT) methods can be used to systematically screen tissue scaffolds to identify optimal biomaterial properties. Previous CHT platforms in tissue engineering have involved a two-dimensional (2D) cell culture format where cells were cultured on material surfaces. However, these platforms are inadequate to predict cellular response in a three-dimensional (3D) tissue scaffold. We describe a simple CHT platform to screen cell-material interactions in 3D culture format that can be applied to screen hydrogel scaffolds. Herein we focus on a method to prepare gradients in elastic modulus of photopolymerizable hydrogels.
Citation: Methods In Bioengineering
Publisher Info: Artech House, Norwood, MA
Pub Type: Books
combinatorial methods, high-throughput screening, hydrogels, elastic modulus, gradients, osteoblast, tissue engineering