Preparation and Value Assignment of Standard Reference Material 968e Fat-Soluble Vitamins, Carotenoids, and Cholesterol in Human Serum

Published

January 2, 2012

Author(s)

Jeanice M. Brown Thomas, David Duewer, Lane Sander, Katherine Sharpless, Lorna T. Sniegoski, Michael J. Welch, James Yen, Mugenya O. Isaac

Abstract

Standard Reference Material 968e Fat-Soluble Vitamins, Carotenoids, and Cholesterol in Human Serum provides certified values for total retinol, γ- and α-tocopherol; total lutein, total zeaxanthin, total β-cryptoxanthin, total β-carotene, and cholesterol in human serum. Values are also reported for nine additional compounds including total α-cryptoxanthin, trans- and total lycopene, total α-carotene, trans-β-carotene, and coenzyme Q10. The certified values for the fat-soluble vitamins and carotenoids in SRM 968e were based on the agreement of results from the means from two liquid chromatographic methods used at the National Institute of Standards and Technology (NIST) and from the median of results from an interlaboratory comparison exercise among institutions that participate in the NIST Micronutrients Measurement Quality Assurance Program. The assigned values for cholesterol in the SRM are the means of results obtained using the NIST reference method, based upon gas chromatography-isotope dilution mass spectrometry. SRM 968e is currently the only available health-related reference material with concentration values assigned for selected retinol, tocopherols, carotenoids, and cholesterol in a human serum matrix. This SRM is used extensively by laboratories worldwide primarily to validate methods for determining these analytes in human serum and plasma and for assigning values to in-house control materials. The value assignment of the analytes in this SRM will help support measurement accuracy and traceability for laboratories performing health-related measurements in the clinical and nutritional communities.

Citation

Analytical and Bioanalytical Chemistry

Volume

402

Pub Type

Journals

Download Paper

Local Download

Keywords

Standard Reference Material, fat-soluble vitamins, carotenoids, frozen human serum, cholesterol, value assignment

Citation

Brown Thomas, J. , Duewer, D. , Sander, L. , Sharpless, K. , Sniegoski, L. , Welch, M. , Yen, J. and Isaac, M. (2012), Preparation and Value Assignment of Standard Reference Material 968e Fat-Soluble Vitamins, Carotenoids, and Cholesterol in Human Serum, Analytical and Bioanalytical Chemistry, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=907947 (Accessed May 12, 2026)

Additional citation formats