Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Using surface plasmon resonance imaging to probe dynamic interactions between cells and extracellular matrix

Published

Author(s)

Alexander W. Peterson, Michael W. Halter, Alessandro Tona, Kiran Bhadriraju, Anne L. Plant

Abstract

Details of cellular interaction with a fibronectin coated surface were examined using surface plasmon resonance imaging (SPRI). SPRI is a label-free highly sensitive technique that is based on the measurement of interfacial refractive index. SPRI is uniquely sensitive to short range interactions between cells and their substrate. With this novel technique, we demonstrate visualization of cell-substrate interactions and how cell-substrate interactions change over time. The high contrast in SPR signal between cell edges and substrate facilitates identification of cell edges and segmentation of cell areas. SPRI was used to follow live cells as they spread, migrated, and exhibited membrane ruffling.
Citation
Cytometry Part A
Volume
77A
Issue
9

Citation

Peterson, A. , Halter, M. , Tona, A. , Bhadriraju, K. and Plant, A. (2010), Using surface plasmon resonance imaging to probe dynamic interactions between cells and extracellular matrix, Cytometry Part A, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=903817 (Accessed March 28, 2024)
Created July 13, 2010, Updated February 19, 2017