Schaffter, S.
, Piorino Macruz de Oliveira, F.
and Romantseva, E.
(2026),
Tunable protein expression strength with toehold exchange riboregulators, Nucleus Engineering, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=963111, https://devnotes.nucleus.engineering/articles/ss-20260712 (Accessed October 25, 2026)
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Tunable protein expression strength with toehold exchange riboregulators
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Abstract
Toehold exchange (THE) riboregulators are a class of RNA molecules that use toehold-mediated strand exchange (TMSE) to regulate protein translation. The mechanism of TMSE offers many design parameters for precisely tuning the strength of protein expression such as: (1) Modulating the concentration of reactants (transcriptional control), (2) Modulating the rate and extent of reaction to produce products (post-transcriptional control), and (3) Modulating the translation rate of the products (translational control). This Developer Note presents detailed, step-by-step protocols for executing translational control experiments for THE riboregulators in cell-free expression systems.Citation
Nucleus Engineering
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Created July 19, 2026, Updated October 8, 2026