NISTCHO Cell Culture and Fed-Batch Bioreactor Protocols

Published

September 24, 2026

Author(s)

Ioannis Karageorgos, William O'Dell, Vamsi Bolla, Hussain Dahodwala, Hussain Dahodwala, Zvi Kelman, John Marino

Abstract

The NISTCHO cell system (RM 8675) provides a standardized Chinese hamster ovary (CHO) cell culture platform for studies of recombinant monoclonal antibody production and bioprocess characterization. Reproducible recovery, expansion, cultivation, and monitoring of NISTCHO cells are important for generating materials with controlled and documented process histories. This Special Publication describes an integrated upstream workflow beginning with recovery of cryopreserved NISTCHO cells and continuing through automated cell concentration and viability measurement, preparation of chemically defined feed media, seed-train expansion, and fed-batch cultivation in a single-use stirred-tank bioreactor. The procedures define operating conditions for temperature, agitation, dissolved oxygen, pH, feeding, glucose supplementation, antifoam addition, sampling, harvest, and initial clarification. A representative 14-day process profile is included to illustrate typical NISTCHO culture behavior. The procedures are intended to facilitate reproducible implementation of the NISTCHO upstream process and generation of cNISTmAb-containing harvest material for downstream processing and analytical studies.

Citation

Special Publication (NIST SP) - 1200-32

Report Number

1200-32

NIST Pub Series

Special Publication (NIST SP)

Pub Type

NIST Pubs

Download Paper

https://doi.org/10.6028/NIST.SP.1200-32

Local Download

Keywords

Bioprocessing, cNISTmAb, Chinese Hamster Ovary Cells, Cell Culture, Fed-batch, Monoclonal Antibody, NISTCHO.

Citation

Karageorgos, I. , O'Dell, W. , Bolla, V. , Dahodwala, H. , Dahodwala, H. , Kelman, Z. and Marino, J. (2026), NISTCHO Cell Culture and Fed-Batch Bioreactor Protocols, Special Publication (NIST SP), National Institute of Standards and Technology, Gaithersburg, MD, [online], https://doi.org/10.6028/NIST.SP.1200-32, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=962965 (Accessed September 25, 2026)

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