Developmental Validation of the PowerPlex® 35GY System: An 8-Dye STR Multiplex for Human Identification Applications

Published

June 22, 2024

Author(s)

Erica Graham, Michael Lauck, John Picciano, Jessica Seifarth, Jon Drobac, Megan O'Donnell, Nisha Sullivan, Lilliana Moreno, Monica Zaky, Lori Flanagan, Caroline Parker, Peter Vallone, Carolyn Steffen, Jesus Martinez, Kristy Lenz, Stephen McDaniel, Dawn Rabbach, Karthika Divakaran, Robert McLaren, Douglas Storts, Cindy Sprecher, Jonelle Thompson

Abstract

The PowerPlex® 35GY System is a 35-locus, eight-dye, STR multiplex optimized for use with Spectrum CE Systems. It simultaneously amplifies all CODIS and ESS markers, along with Amelogenin and DYS391 for gender determination. Penta D, Penta E, and SE33 are included for expanded database searching capability. Ten additional nonrapidly mutating Y-STR loci are included to enable familial searching and to assist with forensic casework on sexual assault evidence. Lastly, two Quality Indicators are added to provide indications of sample quality and amplification performance. The PowerPlex® 35GY System is designed to amplify DNA from purified extracts as well as direct amplification of database sample substrates. The availability of 8 colors allows the inclusion of smaller, more numerous loci, thus increasing a laboratory's chance of success with challenging samples. A multilaboratory developmental validation study was performed for the PowerPlex® 35GY System following SWGDAM guidelines. The results of the study demonstrate the reliability and robustness of the PowerPlex® 35GY System for use in human identification DNA typing applications.

Citation

Forensic Genomics

Volume

4

Issue

2

Pub Type

Journals

Download Paper

https://doi.org/10.1089/forensic.2024.0006

Local Download

Keywords

PowerPlex®, 35GY, 8-dye, Validation, Spectrum, Short Tandem Repeat, Forensic Science, DNA Typing, Challenging Samples

Citation

Graham, E. , Lauck, M. , Picciano, J. , Seifarth, J. , Drobac, J. , O'Donnell, M. , Sullivan, N. , Moreno, L. , Zaky, M. , Flanagan, L. , Parker, C. , Vallone, P. , Steffen, C. , Martinez, J. , Lenz, K. , McDaniel, S. , Rabbach, D. , Divakaran, K. , McLaren, R. , Storts, D. , Sprecher, C. and Thompson, J. (2024), Developmental Validation of the PowerPlex® 35GY System: An 8-Dye STR Multiplex for Human Identification Applications, Forensic Genomics, [online], https://doi.org/10.1089/forensic.2024.0006, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=957718 (Accessed February 20, 2026)