Siegall, W.
and Kelman, Z.
(2024),
Important consideration when expressing mAbs in Escherichia coli, Protein Expression and Purification, [online], https://doi.org/10.1016/j.pep.2024.106499, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=936482
(Accessed October 9, 2025)
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Important consideration when expressing mAbs in Escherichia coli
Published
Author(s)
William Siegall,
Abstract
Monoclonal antibodies (mAbs) are a driving force in the biopharmaceutical industry. The production of therapeutic mAbs is primarily done in mammalian cells, but recently there has been a push towards the use of alternative production hosts including Escherichia coli. When the genes encoding for the mAbs heavy and light chains are codon-optimized for E. coli expression it has been noted that a truncated form of the heavy chain can be formed along with the full-length product. Here, the observation of the factors that lead to a truncated product was expended to several therapeutic mAbs and optimization algorithms. Approaches to avoid this truncated form are discussed.Citation
Protein Expression and Purification
Pub Type
Journals
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Keywords
E. coii, Protein expression, Codon optimization
Citation
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Created May 2, 2024, Updated August 1, 2025