High separation efficiency of gold nanomaterials of different aspect ratio and size using capillary transient isotachophoresis

Published

August 2, 2019

Author(s)

Kathryn R. Riley, Hind El Hadri, Jiaojie Tan, Vincent A. Hackley, William A. MacCrehan

Abstract

Capillary electrophoresis (CE) has shown considerable promise for the analysis of sub-micron and nanoscale materials. However, many proposed methods utilize expensive coated capillaries or additives, like surfactants, to achieve high peak efficiency and resolution. Here we report the development of capillary transient isotachophoresis, ctITP, as an on-line technique for the improved detection and separation of gold nanomaterials. Overall, an approximate 2-fold decrease in peak width was observed for citrate-stabilized gold nanoparticles/rods and CTAB- coated gold nanorods relative to traditional capillary zone electrophoresis (CZE). Further, gold nanorods differing by aspect ratio that could not be separated by other techniques (e.g. single particle inductively coupled plasma mass spectrometry, sp-ICP-MS, and asymmetric flow field-flow fractionation, A4F) were baseline resolved using ctITP.

Citation

Journal of Chromatography A

Volume

1598

Pub Type

Journals

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DOI Link

Keywords

capillary electrophoresis, capillary transient isotachophoresis, gold nanoparticles, gold nanorods, field flow fractionation, inductively coupled plasma mass spectrometry

Citation

Riley, K. , El Hadri, H. , Tan, J. , Hackley, V. and MacCrehan, W. (2019), High separation efficiency of gold nanomaterials of different aspect ratio and size using capillary transient isotachophoresis, Journal of Chromatography A, [online], https://doi.org/10.1016/j.chroma.2019.03.054 (Accessed October 9, 2025)