MICROFABRICATION OF A 3D, ELASTIC CELL MICROENVIRONMENT WITH NANOSCALE PRECISION USING LAYER-BY- LAYER DEPOSITION

Published

October 22, 2017

Author(s)

Markela Ibo, Kiran Bhadriraju, Darwin Reyes-Hernandez

Abstract

Here we present a unique method to generate a customized cell microenvironment that allows for the positioning of specific components of the extracellular matrix, and other polyelectrolytes, at a precise distance from the matrix surface; thus, providing a variable, well-coordinated material that could be tailored for essential cell-surface interactions. Weakly cross-linked melamine formaldehyde (MF) microparticles were packed in microfluidic channels and polyelectrolyte multilayers were formed, layer-by-layer, on top of them. The MF microparticles were then dissolved and matrices with an elastic modulus of 10 kPa, and capable of maintaining cells alive for at least 24 h, were obtained.

Proceedings Title

Proceedings of Micro Total Analysis Systems 2017

Conference Dates

October 22-26, 2017

Conference Location

Savannah, GA, US

Conference Title

The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2017
Conference

Pub Type

Conferences

Keywords

Layer-by-layer deposition, 3D-like cell culture matrix, laminin, fibronectin, polyelectrolytes

Citation

Ibo, M. , Bhadriraju, K. and Reyes-Hernandez, D. (2017), MICROFABRICATION OF A 3D, ELASTIC CELL MICROENVIRONMENT WITH NANOSCALE PRECISION USING LAYER-BY- LAYER DEPOSITION, Proceedings of Micro Total Analysis Systems 2017, Savannah, GA, US (Accessed April 30, 2024)