State-of-the-art and trends for the SI traceable value assignment of the purity of peptides using the model compound angiotensin I

Published

March 27, 2018

Author(s)

Ralf D. Josephs, Norbert Stoppacher, Adeline Daireaux, Tiphaine Choteau, Katrice Lippa, Karen W. Phinney, Steven Westwood, Robert Wielgosz

Abstract

Accurate diagnosis, monitoring and treatment are cornerstones of a healthcare system and require reliable measurements. The traceability of measurement results to stated references, and the development of Reference Measurement Systems (RMS), provide mechanisms to achieve the required consistency of results. The primary standards within such an RMS are of pure substance certified reference materials. Developments in modern measurement and mass spectrometric techniques have opened up the possibility to characterize and quantify more complex large molecules such as peptides, enabling RMS to be developed for these analytes. The prohormone angiotensin I is a hypertension biomarker and was selected as a model peptide. The full mass balance approach, the peptide impurity corrected amino acid analysis, elemental analysis and quantitative nuclear magnetic resonance approaches have been investigated and optimized to compare the performance of these different methodologies for the purity mass fraction value assignment of an ANG I calibrator material.

Citation

Trac-Trends in Analytical Chemistry

Volume

101

Pub Type

Journals

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DOI Link

Keywords

Angiotensin, peptide, impurity, purity, uncertainty, traceability

Citation

Josephs, R. , Stoppacher, N. , Daireaux, A. , Choteau, T. , Lippa, K. , Phinney, K. , Westwood, S. and Wielgosz, R. (2018), State-of-the-art and trends for the SI traceable value assignment of the purity of peptides using the model compound angiotensin I, Trac-Trends in Analytical Chemistry, [online], https://doi.org/10.1016/j.trac.2017.09.026 (Accessed October 9, 2025)