Ripple, D.
and Narhi, L.
(2015),
Protein Particles (0.1 υm to 100 υm), ACS Publications, Washington, DC, [online], https://doi.org/10.1021/bk-2015-1201.ch008
(Accessed April 23, 2024)
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Protein Particles (0.1 υm to 100 υm)
Published
Author(s)
, Linda Narhi
Abstract
Protein molecules in solution can form proteinaceous particles by a variety of aggregation processes. The size and concentration of these particles is an important quality attribute for therapeutic MAb solutions. In this chapter, we describe the techniques commonly used to determine size and count of particles in solution for the size range 1 µm to 100 µm. After first discussing general principles of particle formation and properties, we present general guidance on sample handling and particle measurement, and then give detailed guidance on the application of the two most common techniques: light obscuration and flow imaging. The chapter concludes with a description of advanced techniques suitable for measurement of these particles.Citation
State-of-the-Art and Emerging Technologies for Therapeutic Monoclonal Antibody Characterization Volume 2. Biopharmaceutical Characterization: The NISTmAb Case Study
Publisher Info
ACS Publications, Washington, DC
Pub Type
Books
Download Paper
Keywords
aggregation, flow microcopy, flow imaging, light obscuration, mab, monoclonal antibody, particle, protein
Citation
Created October 15, 2015, Updated November 10, 2018