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Biosafety Level 2 Facility

This facility provides equipment and protocols to culture and evaluate animal and human cell lines and tissue, which are essential aspects of several of our research projects. We also maintain the facility for access by other users on campus. Equipment includes 3 Class II/B2 fully vented biosafety cabinets, swinging bucket and fixed rotor centrifuges, water-jacketed COincubators, and cell storage in an ultra-low freezer (-80 °C) or LN2 storage dewar (-160 °C).


Analytical instruments:
       Phase contrast microscope
       Microvolume UV-VIS spectrophotometer
       Epifluorescence microscope
       Plate reader for absorbance, fluorescence, and luminescence-based assays
       Dynamic light scattering

Transmission and Fluorescence
Evidence that the odorant receptor MOR244-3 is expressed at the surface of Hana3A cells was obtained by live cell immunocytochemical labeling of mouse monoclonal anti-rhodopsin antibody (4D2). Transmission image (left) shows cell density while fluorescence image (right) reveals discrete points of high fluorescence intensity suggesting surface protein expression.

We use cells as a tool to study thermodynamic properties in complex matrices. We have experience culturing immortalized cell lines and primary cells including Hana 3A (human embryonic kidney), HeLa (human cervical cancer), SW-13 (human adrenal cortex), NPC (rat cortical neural progenitor cells), PC-12 (rat pheochromocytoma), NIH 3T3 (murine embryonic fibroblasts), and RAW 264.7 (murine macrophage).

Past and current projects include:
        Partitioning behavior of drug compounds and metabolites in human blood
        Activation and deactivation of cannabinoid receptors by nuclear magnetic resonance (NMR) spectroscopy screening
        Role of metals in binding to odorant receptors


Facility Manager

Created June 10, 2020, Updated June 12, 2020