isoelectric focusing (IEF)
A method of separating amphoteric molecules with minor differences in isoelectric points (pI). IEF can be used with several different enzyme systems including Phosphoglucose Isomerase (PGI), Erythrocyte Acid Phosphatase (EAP), Superoxide Dismutase (SOD) or Tetrazolium Oxidase (TO), Albumin (in conjunction with Western Blot), Mannose Phosphate Isomerase (MPI), Carbonic Anhydrase I (CAI) and Hemoglobin (Hb).
Committee
Wildlife Forensic Biology
Date Added to Lexicon
Standard Source (Designation Number Title of Standard. Publisher, Place, Year.)
ANSI/ASB 106 Wildlife Forensics—Protein Serology Method for Taxonomic Identification. AAFS Academy Standards Board (ASB), 410 North 21st Street, Colorado Springs, CO, 2020.
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