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Back to the OSAC Lexicon

Sanger Sequencing

A method of DNA sequencing for determining the order of bases in a DNA molecule based on the selective incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication. See sequencing below.

Date Added to Lexicon
Standard Source (Designation Number Title of Standard. Publisher, Place, Year.)
ANSI/ASB 047 Wildlife Forensics Validation Standard - Validating New Primers for Sequencing. AAFS Academy Standards Board (ASB), 410 North 21st Street, Colorado Springs, CO 80904, 2019. Source link
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