Gene delivery systems

viral vector illustration with RNA seen within the capsid

Gene Delivery Particle Analysis with High-Throughput Light Scattering Microscopy

Ongoing

This project aims to develop light-scattering microscopy methods for rapid, multi-attribute characterization of gene therapy particles at the single particle level. Emerging gene therapy treatments use gene delivery particles, such as viral vectors and non-viral carriers, to cure intractable

High-Sensitivity IR Spectroscopy of Proteins

Ongoing

Infrared (IR) absorption spectroscopy has been widely used as a non-invasive, label-free characterization method for the chemical identification and structure of complex biomolecules. Fourier-transform IR (FT-IR) technology is commonly used to characterize proteins and other biological molecules

Cell Viability for Advanced Therapies

Ongoing

In the Biosystems and Biomaterials Division at NIST, we have established several technical programs to support the development and implementation of cell viability assays in advanced therapies. These programs address key needs in cell viability assay development including selection and development

Integrated Lentiviral Vector Copy Number Measurements: Invitation to Participate in an Interlaboratory Testing Program Evaluating Genomic DNA Test Materials

Ongoing

NIST has produced genomic DNA test materials from transduced Jurkat cell lines with integrated lentiviral vector copy number (VCN) for evaluation as a candidate reference material. Transducing Jurkat (Clone E6-1) cell lines with a lentivirus expressing a green fluorescent protein were produced by

Gene delivery systems

The Research