Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Using Self-Assembly to Control the Structure of DNA Monolayers on Gold: A Neutron Reflectivity Study

Published

Author(s)

R Levicky, T M. Herne, Michael J. Tarlov, Sushil K. Satija

Abstract

Neutron reflectivity was used to determine the concentration profiles of oligomeric DNA monolayers on gold in the high salt (1M NaCl). These monolayers are of interest as model DNA probe systems used in diagnostic devices. To facilitate its attachment, the DNA was functionalized at the 5' end with a thiol group connected to the oligonucleotide by a hexamethylene linker. Concentration profiles determined from neutron reflectivity indicate that adsorbed layers of single-stranded DNA (HS-ssDNA) on bare gold are compact, suggesting the presence of multiple contacts between each DNA strand and the surface. After treatment with mercaptohexanol, a short alkanethiol with a terminal hydroxy group, the DNA stands-up and extends farther into the solvent phase. These changes are consistent with the DNA remaining attached through its thiol end group while contacts between DNA backbones and the surface are prevented by the formation of a mercaptohexanol monolayer. The end-tethered HS-ssDNA layer readily hybridized to its complementary sequence, resulting in DNA helices with a preferred orientation toward the substrate normal.
Citation
Journal of the American Chemical Society
Volume
120
Issue
No. 38

Keywords

alkanethiol monolayer, brush, DNA, geonomics, neutron reflection, polymer, self-assembly, structure, surface

Citation

Levicky, R. , Herne, T. , Tarlov, M. and Satija, S. (1998), Using Self-Assembly to Control the Structure of DNA Monolayers on Gold: A Neutron Reflectivity Study, Journal of the American Chemical Society (Accessed July 19, 2024)

Issues

If you have any questions about this publication or are having problems accessing it, please contact reflib@nist.gov.

Created September 30, 1998, Updated February 17, 2017