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Using Raman Imaging to Detect Oxidative Stress Events in Plant Tissues

Published

Author(s)

B M. Allen, Anne L. Plant

Abstract

Raman Imaging represents the union of Raman spectroscopy and optical microscopic imaging. This non-invasive vibrational scattering technique allows one to simultaneously spatially (approximately} 1 micron) and spectrally (approximately} 7 wavenumbers) resolve subcellular biological components. Unique Raman signatures of various molecular components of plant chloroplasts will be monitored in response to exposure to excessive amounts of ozone, in the presence and absence of an anti-ozonant commonly known as EDU. Chloroplasts house most of the photosynthetic apparatus of plant tissues and it is the attempt of this study to ascertain the compositional, organizational and possibly conformational changes that occur here in response to excessive ozone exposure. This technique will be evaluated for its potential as a measurement tool of oxidative stress events with representative in-vivo and in-situ samples.
Citation
Using Raman Imaging to Detect Oxidative Stress Events in Plant Tissues

Keywords

chloroplasts, oxidative stress, Raman imaging

Citation

Allen, B. and Plant, A. (1999), Using Raman Imaging to Detect Oxidative Stress Events in Plant Tissues, Using Raman Imaging to Detect Oxidative Stress Events in Plant Tissues (Accessed May 22, 2024)

Issues

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Created January 29, 1999, Updated October 12, 2021