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The Use of Real-Time Polymerase Chain Reaction in Quantifying Inflammatory Responses to Biomaterials

Published

Author(s)

Washburn N. BaileyOL, Carl Simon Jr., Wang F. ChanSE

Abstract

: The introduction of tissue engineering approaches for the repair and replacement of human body components extends the application and importance of synthetic materials (biomaterials). Implanted biomaterials frequently evoke inflammatory responses, and are subsequently responsible for implant failure. We have established a system that allows for the analysis and quantitation of cellular inflammatory responses in vitro using Real- Time Polymerase Chain Reaction (RT-PCR). The inflammatory responses of non-transformed murine macrophages (Raw 264.7) cells were analyzed after incubation with polymethylmethacrylate (PMMA) microspheres in the presence and absence of lipopolysaccharide (LPS) at 8hrs. Elevated cytokine production serves as an indicator of inflammatory responses, thus we monitored the levels of interlukin-1b produced. Furthermore, we demonstrate that the presence of a bacterial component and debris particles can work in concert to elevate cytokine production leading to inflammation.
Citation
Society for Biomaterials Meeting
Volume
19(8)

Keywords

tissue engineering

Citation

BaileyOL, W. , Simon Jr., C. and ChanSE, W. (2003), The Use of Real-Time Polymerase Chain Reaction in Quantifying Inflammatory Responses to Biomaterials, Society for Biomaterials Meeting, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=852824 (Accessed February 24, 2024)
Created December 31, 2002, Updated October 12, 2021