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Universal Gradient Substrates for Click Biofunctionalization



Nathan D. Gallant, Kristopher Lavery, Eric J. Amis, Matthew Becker


In order to facilitate research on biomimetic and tissue engineered medical products we have developed a novel and versatile method for fabricating continuously variable concentration gradients of surface conjugated biomolecules. This technology utilizes graded UV oxidation of self-assembled monolayers (SAMs) and upon further derivatization, converts the resulting carboxylate gradient into an increasing density of alkyne functionality appropriate for click chemistry surface conjugation of biomolecules. Thus, any ligand engineered with an accessible azo group may be immobilized into the surface concentration gradient, which we have therefore named a Universal Gradient Substrate for Click Biofunctionalization . This combinatorial approach, coupled with high throughput analysis techniques such as automated fluorescence microscopy, forms the basis of a platform for screening the ligand density dependence of cell response to biomaterials. In the current study this method has been applied to generate a gradient of an arginine-glycine-aspartate (RGD) peptide to control cell adhesion, and the design, fabrication, and characterization is described.
Advanced Materials


biomaterials, biomimetic gradient, cell adhesion, click chemistry, combinatorial methods, RGD peptide


Gallant, N. , Lavery, K. , Amis, E. and Becker, M. (2006), Universal Gradient Substrates for Click Biofunctionalization, Advanced Materials, [online], (Accessed July 21, 2024)


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Created February 22, 2006, Updated February 17, 2017