A Uniaxial BioMEMS Device for Quantitative Force-Displacement Measurements
David Serrell, Tammy L. Oreskovic, Andrew Slifka, Roop L. Mahajan, Dudley Finch
There is a need for experimental techniques that allow the simultaneous imaging of cellular cystoskeletal components with quantitative force measurements on single cells. A bioMEMS device has been developed for the application of strain to a single cell while simultaneously quantifying its force response. The prototype device presented here allows the mechanical study of a single, adherent cell in vitro. It works in a similar fashion to a displacement-controlled uniaxial tensile machine, and is calibrated using an AFM cantilever demonstrating excellent agreement with the theoretical spring constant. A single fibroblast is used here to illustrate how the device operates. The force response of the cell is seen to be linear until the onset of de-adhesion with the cell de-adhering from the cell platform at a force of approximately 1500 nN.
, Oreskovic, T.
, Slifka, A.
, Mahajan, R.
and Finch, D.
A Uniaxial BioMEMS Device for Quantitative Force-Displacement Measurements, Biomedical Microdevices, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=50343
(Accessed February 20, 2024)